Table 1 Cas9 variants that have been modified to broaden the scope of application
Variant name | Resources (PAM) | Selection strategy | PAM | Ref. |
|---|---|---|---|---|
spCas9-NG | SpCas9 (NGG) | Elimination of the interaction between Cas9 and the third position of PAM | NG | |
xCas9 | SpCas9 | PACE | NG, GAA, GAT | |
Cas9-NRNH | SpCas9 | PANCE and PACE that evolved by enabling SpCas9 to bind to specific sequences with non-G PAMs | NRNH | |
SpG | SpCas9 | Structure-guided engineering | NGN | |
SpRY | SpCas9 | Structure-guided engineering | Almost unlimited | |
Cas9-VQR | SpCas9 | Bacterial selection system | NGAN NGCG | |
Cas9-EQR | SpCas9 | Bacterial selection system | NGAG | |
SaCas9-KKH | SaCas9 (NNGRRT) | Molecular evolution and bacterial selection system | NNNRRT | |
eNme2-C | NmCas9 (NNNNGATT) | PANCE, ePACE, and BE-PPT | Almost unlimited | |
eNme2-C.NR | NmCas9 | PANCE, ePACE, and BE-PPT | Almost unlimited | |
eNme2-T.1 | NmCas9 | PANCE, ePACE, and BE-PPT | NTN | |
eNme2-T.2 | NmCas9 | PANCE, ePACE, and BE-PPT | NTN |
