Table 3 Characteristics of various vectors loaded with the CRISPR/dCas9 system
Delivery vector | Advantages of delivering CRISPR systems | Disadvantages of delivering CRISPR systems | Ref. |
|---|---|---|---|
AAV vector | High delivery efficiency, security, clinical certification | Limited packaging capacity, high cost, pathogenicity | |
Lentivirus vector | High delivery efficiency, low immunogenicity | High cost, construction is difficult | |
Baculovirus vector | Large packaging capacity, low genotoxicity | Limited delivery efficiency | |
Lipid-based nanoparticles | Easy to transform, security, large packaging capacity | Easily ingested by the liver | |
Polymer-based nanoparticles | Easy to transform, controllable release | Complicated packaging construct, limited application | |
Natural exosomes | High biocompatibility, easy access | Easily ingested by the liver | |
Functional exosomes | High biocompatibility, high specificity | Medium delivery efficiency, easily ingested by the liver | |
AuNPs | Easy to transform, anti-inflammatory properties, special physicochemical properties, large packaging capacity | High cost | |
Biomimetic nanoparticles | High biocompatibility, easy to transform, anti-inflammatory properties, large packaging capacity | Limited delivery efficiency |
