Fig. 1
SIRT7 is a stress-responsive factor and significantly up-regulated in melanoma. a Relative mRNA fold change of all sirtuins family members in A2058 cells treated with TM (3 μM) or HBSS for 24 h. b Immunoblotting analysis of SIRT7 expression in A2058 and A375 cells treated with TM (3 μM), TG (1 μM), 2-DG (10 mM), HBSS or H2O2 (400 μM) for 24 h. c Immunofluorescence staining of SIRT7 and Ac-H3K18 in A2058 cells treated with TM (3 μM) or HBSS for 24 h. Scale bar, 50 μm. d Representative pathways associated with SIRT7 expression in TCGA SKCM database. e GSEA analysis between SIRT7 expression and reactive oxygen species, unfolded protein response, P53 pathway and BIOCARTA stress pathway in TCGA SKCM database and 88 melanoma cell lines short-term cultures database. f Relative mRNA level of SIRT7 in melanoma cell lines and two cultures of HPMs. g, h Immunoblotting analysis of SIRT7 expression and quantified graph in melanoma cell lines and two cultures of HPMs. i, j Immunohistochemical staining analysis of SIRT7 and Ac-H3K18 in TMA. Scale bar, 100 μm. k Correlation analysis of SIRT7 and Ac-H3K18 scores in TMA. l SIRT7 expression in melanoma and nevus in Talantov dataset (GDS1375). m The comparison of the survival between patients with low SIRT7 level and high SIRT7 level in TCGA SKCM database. Data represent the mean ± SD of triplicates. r value was calculated by Spearman correlation. P value was calculated by two tailed Student’s t-test, *P < 0.05, **P < 0.01, ***P < 0.001, HPM#1 group as control; #P < 0.05, ##P < 0.01, ###P < 0.001, HPM#2 group as control. ns non-significant
