Fig. 2 | Signal Transduction and Targeted Therapy

Fig. 2

From: SIRT7 orchestrates melanoma progression by simultaneously promoting cell survival and immune evasion via UPR activation

Fig. 2

SIRT7 enables melanoma cell survival under stress. a, b Cell viability and apoptosis rate of A2058 and A375 melanoma cells treated with TM (3 μM) or HBSS for 24 h with or without the knockdown of SIRT7. c Colony formation of A2058 cells treated with TM (3 μM) or HBSS for 24 h with or without the knockdown of SIRT7. d Immunoblotting analysis of apoptosis-related molecules expression in A2058, A375 and WM35 cells with indicated treatment. e Images of A2058 xenografts with indicated treatment isolated from nude mice. Tumor volumes and weights in each group were calculated and displayed on the right. Symbols of one dot indicates one mouse, and the error bars are mean with ±S.D. f Immunofluorescence staining of SIRT7 and HSPA5 in tumors from nude mice. Scale bar, 50 μm. g Immunofluorescence staining of ki67 and cleaved-caspase3 in tumors from nude mice. Scale bar, 50 μm. Data represent the mean ± SD of triplicates. P-value was calculated by two-tailed Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001

Back to article page