Fig. 5
Regulation of SRSF1 on UBE2B-exon7-SE in tuberculosis. a, b qRT-PCR results showing the UBE2B-exon7-SE splicing and SRSF1 mRNA expression in macrophages with and without HKMT stimulation, respectively. (n = 3 biologically independent samples). Data are represented as mean ± standard deviation. Padj was calculated by adjusting the splicing state of macrophages at the 0 h time point. c Western blotting results showing SRSF1 level in macrophages with and without HKMT stimulation. d Immunofluorescence staining of SRSF1 (red) and DAPI (blue) in macrophages. Scale bars for the images are 20 μm. e RNA immunoprecipitation showing the interaction between SRSF1 and UBE2B sequence. (n = 3 biologically independent samples). Data are represented as mean ± standard deviation. f RNA immunoprecipitation showing the alternations of UBE2B-SRSF1 binding caused by HKMT stimulation. (n = 3 biologically independent samples). g, h qRT-PCR results showing the splicing of UBE2B-exon7-SE in HKMT-stimulated control and SRSF1-OE, as well as shControl and shSRSF1. (n = 3 biologically independent samples). Data are represented as mean ± standard deviation. i Heatmaps showing the expression of differential ubiquitination proteins. j KEGG enrichment analysis of differential ubiquitination proteins. This plot showed the top 30 enriched pathways. k Bar plots showing the expression of differential ubiquitination proteins enriched in the apoptosis pathway. Data are represented as mean ± standard deviation. SRSF1 serine/arginine-rich splicing factor 1, UBE2B Ubiquitin Conjugating Enzyme E2 B, SE skipping exon, PSI percent spliced in, HKMT heat-killed mycobacterium tuberculosis, OE overexpression
