Fig. 2 | Signal Transduction and Targeted Therapy

Fig. 2

From: Phase-separated nucleocapsid protein of SARS-CoV-2 suppresses cGAS-DNA recognition by disrupting cGAS-G3BP1 complex

Fig. 2

SARS-CoV-2 infection activates cGAS-mediated IFN-I signaling. a Immunoblot analysis of GFPsgRNA and cGASsgRNA Calu3 cells infected with SARS-CoV-2 (MOI = 0.1) for indicated time points. b qRT-PCR with reverse transcription analysis of cGAS, IFNβ, IFIT1, and IFIT2 mRNA level of GFPsgRNA and cGASsgRNA Calu3 cells with SARS-CoV-2 (MOI = 0.1) infection for indicated time points. c Cellular IFNβ production of GFPsgRNA and cGASsgRNA Calu3 cells infected with SARS-CoV-2 (MOI = 0.1) for indicated time points detected by ELISA assay. d qRT-PCR with reverse transcription analysis of N protein and ORF1ab RNA of SARS-CoV-2 in supernatant of GFPsgRNA and cGASsgRNA Calu3 cells with SARS-CoV-2 (MOI = 0.1) infection for indicated time points. e Cellular cGAMP production of GFPsgRNA and cGASsgRNA Calu3 cells infected with SARS-CoV-2 (MOI = 0.1) for indicated time points detected by ELISA assay. Data in b–e were expressed as mean ± SEM of 3 independent biological experiments. ***P < 0.001, ****P < 0.0001, ns not significant (unpaired two-tailed student’s t-test). Similar results were obtained for 3 independent biological experiments in a

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