Fig. 2 | Signal Transduction and Targeted Therapy

Fig. 2

From: Soluble CD4 effectively prevents excessive TLR activation of resident macrophages in the onset of sepsis

Fig. 2

CD4+ T promoted monocytes differentiation and dampened resident macrophage hyperinflammation. a–c, e–i Nude mice or nude mice reconstituted with CD4+ T cells were treated i.p. LPS for 3 h before indicated analysis. Cell numbers of (a) Ly6Chi monocytes, (b) progenitor cells in BM (HSC, GMP, CMP, MEP), and (c) activated monocytes (CD64+) in the indicated organs were flow cytometric analyzed. d Proportions of indicated cell types after in vitro differentiation of BM-derived monocytes co-cultured with CD4+ T cells, for the indicated time of LPS treatment. Cell numbers of (e) infiltrated monocyte derived macrophages or (g) resident macrophages and (f, h) their activation status (CD86+). i TNF mRNA induction by LPS in the indicated cell types sorted from the spleen. Mean ± SD are shown; n = 3–5 mice used where indicated; Statistics (ns, P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001): Unpaired t test

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