Fig. 5 | Signal Transduction and Targeted Therapy

Fig. 5

From: Reduced smooth muscle-fibroblasts transformation potentially decreases intestinal wound healing and colitis-associated cancer in ageing mice

Fig. 5

Ageing reduces fibroblasts derived from the ex-vivo culture of the muscular layer. a SMCs derived cells (SDCs) from the muscular layer of young and ageing mice at day 7 and day 14. Scale bars, 100 μm. b Detection of Vimentin, PCNA and DAPI of SDCs from the muscular layer of young and ageing mice at day 14. Scale bars, 50 μm. c The ratio of Vimentin+ cells and PCNA+ cells in SDCs from the young and ageing muscular layer. n = 5 for each group, Data are mean ± SD. d, e Intestinal organoids derived from 400 crypts cultured alone or 400 crypts cocultured with 10000 SDCs from young or ageing mice, organoids number was measured at day 5, n = 5 for each group, mean ± SD. Scale bars, 100 μm

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