Fig. 6 | Signal Transduction and Targeted Therapy

Fig. 6

From: Reduced smooth muscle-fibroblasts transformation potentially decreases intestinal wound healing and colitis-associated cancer in ageing mice

Fig. 6

Detection of YAP/TAZ in ISMCs during murine intestinal wound healing and in IBD patients. a Detection of the Myh11 and YAP in ISMCs in homeostasis or during the early phase of CAC model in young and ageing mice. Scale bars, 50 μm. b The ratio of YAP-positive cells in muscularis mucosa at A+D-d10 in young and ageing mice. Young, n = 6; Ageing, n = 8; Data are mean ± SD. c Detection of the Myh11 and TAZ in ISMCs at A+D-d10 in young and ageing mice. Scale bars, 50 μm. d Serial sections detection of YAP, Vimentin and Myh11 around muscularis mucosa at A+D-d10 in young and ageing mice. Scale bars, 50 μm. e Pearson’s correlation between the ratio of YAP-positive cells in muscularis mucosa and the ratio of Vimentin+ fibroblasts in stromal cells around the muscularis mucosa at A+D-d10. Pearson r = 0.95, P < 0.0001, n = 15. f Immunohistochemical staining of YAP and Vimentin at A+D-d0 and A+D-d10. Scale bars, 50 μm. g Relative mRNA expression of YAP and TAZ in the muscular layer of young and ageing mice at DSS-d0 and DSS-d10, n = 3 for each group, Data are mean ± SD. h Serial sections immunohistochemical staining of Myh11 and YAP in Crohn’s disease patients. Scale bars, 50 μm. i Serial sections immunohistochemical staining of Myh11 and YAP in ulcerative colitis patients. Scale bars, 50 μm. j Serial sections immunohistochemical staining of Myh11 and TAZ in Crohn’s disease patients. Scale bars, 50 μm. k Serial sections immunohistochemical staining of Myh11 and TAZ in ulcerative colitis patients. Scale bars, 50 μm

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