Fig. 7
YAP/TAZ is required for ISMCs to transform into fibroblasts during intestinal wound healing. a Schematic overview of the tamoxifen induction to conditionally knockdown YAP/TAZ in ISMCs during the early phase of the CAC model. b Detection of Myh11 and YAP in ISMCs of muscularis mucosa at A+D-d10 in Myh11-cre/ERT2(Ctrl) and Myh11-cre/ERT2; YAPfl/fl/TAZfl/+ (cDKO) mice. Scale bars, 50 μm. c The ratio of YAP-positive cells in muscularis mucosae at A+D-d10 in Ctrl and cDKO mice. n = 6, Data are mean ± SD. d SMCs-derived cells (SDCs) from the muscular layer of Ctrl and cDKO mice. Scale bars, 100 μm. e Detection of Vimentin and PCNA in the colon tissues of Ctrl and cDKO mice at A+D-d10. Scale bars, 50 μm. f The ratio of Vimentin+ fibroblasts in intestinal stromal microenvironment at A+D-d10 in Ctrl and cDKO mice. n = 6, mean ± SD. g Survival analysis of cDKO mice (red line; n = 22) compared to that of Ctrl mice (blue line; n = 26; P < 0.001 by log-rank test) during the early phase of the CAC model. h Immunofluorescence images of the temporal re-epithelialization process that occurs during the early phase of the CAC model in Ctrl and cDKO mice. Sections are immunolabeled for Epcam and Myh11. Scale bars, 50 μm. i High-resolution endoscopic images of the colon at A+D-d21 in Ctrl and cDKO mice. j Immunohistochemical staining of p-stat3 in colon tissues at A+D-d21 in Ctrl and cDKO mice. Scale bars, 50 μm. k Representative macroscopic images of CAC in Ctrl and survival cDKO mice at A+D-d63. l Tumor number per colon was measured at A+D-d63. Ctrl, n = 12; cDKO, n = 7, P < 0.05. Data are mean ± SD. m Tumor area per colon was measured at A+D-d63. Ctrl, n = 12; cDKO, n = 7, P < 0.01. Data are mean ± SD
