Table 2 The advantage and disadvantage of the three circularization methods
From: Circular RNA vaccine in disease prevention and treatment
| Â | Advantage | Disadvantage |
|---|---|---|
Chemical synthesis strategies | • No exogenous sequence introduction | • Low circularization efficiency for long linear precursor • Complicated design and synthesis procedures • Complementary template requirement • Low-throughput synthesis • Unknown immunogenicity |
Enzymatic synthesis strategies | • No exogenous sequence introduction • Lowest immunogenicity | • Low circularization efficiency for long linear precursor • Complicated synthesis procedure • Complementary template requirement • Low-throughput synthesis |
PIE system | • Simple synthesis procedure • Suitable for circularization of long linear precursor • Applicable to high-throughput synthesis • Low immunogenicity | • Exogenous sequence introduction (group I intron PIE system) • Contaminants present after synthesis |
