Fig. 4
From: Microcolin H, a novel autophagy inducer, exerts potent antitumour activity by targeting PITPα/β
Identification of PITPα/β as the target of microcolin H. a Structures of analogues 1-5 and probes 1-5. b A cell counting kit-8 (CCK-8) assay was used to analyse the effect of analogues and probes on HGC-27 cell viability. Cells were treated with various doses of probes for 48. c Labelling and pull-down workflow. d Competitive Labelling with microcolin H in HGC-27 cells. The complete gel of Labelling was stained with Coomassie brilliant blue (CBB). e Pull down of HGC-27 cells, CuAAC with “TAMRA-Bioin-Azide” trifunctional reporter, followed by enrichment and in-gel analysis. f Western blot analysis to determine the competitive binding of the probe and microcolin H to PITPα and PITPβ. g MST analysis was used to assess the binding between microcolin H and PITPβ in kinetic level. Data are presented as the mean ± SD
