Fig. 5
From: Microcolin H, a novel autophagy inducer, exerts potent antitumour activity by targeting PITPα/β
Microcolin H functions by binding PITPα/β. a Western blot analysis of PITPα/β expression in wild type (WT) and PITPα/β knockout HGC-27/MKN-28 cells (PITPα/βKO-1/2). b Wild type (WT) and PITPα/β knockout HGC-27/MKN-28 cells (PITPα/βKO-1/2) were treated with various concentrations of microcolin H for 12 h, and cell proliferation was detected by using a CCK-8 assay, n = 3 per group. c Wild type (WT) and PITPα/β knockout HGC-27/MKN-28 cells (PITPα/βKO-1/2) were treated with microcolin H for 48 h. Then, the cells were cultured for 9 d, and the colonies were counted, n = 3 per group. d Western blot analysis of PITPα/β expressions for wild type (WT) and PITPα/β overexpression HGC-27/MKN-28 cells (OV-PITPα/β). e Wild type (WT) and PITPα/β overexpression HGC-27/MKN-28 cells (OV-PITPα/β) were treated with various concentrations of microcolin H for 12 h, and cell proliferation was detected by using a CCK-8 assay, n = 3 per group. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, vs. control group
