Fig. 6
From: Microcolin H, a novel autophagy inducer, exerts potent antitumour activity by targeting PITPα/β
Microcolin H induces autophagy by binding PITPα/β. a The HGC-27/MKN-28 cells were incubated with microcolin H as indicated time or concentration. Whole lysate was used for detecting p62 and LC3 expression using western blot assay. b Western blot analysis of LC3 expression after HGC-27 cells had been treated with microcolin H and HCQ. c Co-IP assay with an antibody against BCL-2 to detect the BCL-2/Beclin-1 interaction in gastric cancer cells treated with microcolin H for 6 h. d HGC-27 cells were treated with 50 nM microcolin H or DMSO and dynamically monitored by a content analyser for 26 h. e A cell counting kit-8 (CCK-8) assay was used to analyse the effect of microcolin H on HCQ/3-MA (25 μM) pre-treated HGC-27 cells viability. Cells were treated with various doses of microcolin H for 7 h, n = 3 per group. f, g HeLa-Difluo™ hLC3 cells and transfected HeLa-Difluo™ hLC3 cells were treated with microcolin H, and autophagosomes were observed by CLSM. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, vs. control group
