Fig. 1
From: Oncolytic adenoviruses expressing checkpoint inhibitors for cancer therapy
Immune cell typing across human and mouse TMEs. a, b The immune landscape was identified by mIHC staining of human glioma, colon cancer, breast cancer and lung cancer tumor microarrays. c, d Heatmap of immune checkpoint molecules in tumors to matched normal expression ratios (log2 (TPM + 1)) (c) and OS of patients with tumors (d). e The expression of CD24, CD47 and CD155 in MC38, 4T1, and CT26 tumor cells was detected by flow cytometry. f C57BL/6J mice were subcutaneously inoculated with 1 × 106 MC38 cells. BALB/c mice were subcutaneously inoculated with 1 × 106 4T1 cells or 1.5 × 106 CT26 cells. When tumor sizes reached ~100 mm3, single-cell suspensions were prepared from the mouse tumors prior to analyzing the composition of immune cells by flow cytometry. Data are represented as mean ± SD
