Fig. 6

EV-packaged circTLCD4-RWDD3 is internalized by HLECs to facilitate lymphangiogenesis. a Representative fluorescence images of HLECs after incubating with PKH67-labeled EVs. Scale bars, 5 μm. b, c qRT-PCR analysis of circTLCD4-RWDD3 expression in HLECs treated with EVs. d qRT-PCR analysis of circTLCD4-RWDD3 and TLCD4-RWDD3 mRNA expression in circTLCD4-RWDD3^KO HLECs. e–g Representative images (e) and quantification of the Transwell migration (f) and tube formation (g) of circTLCD4-RWDD3^WT and circTLCD4-RWDD3^KO HLECs treated with A549-EV_Vector or A549-EV_{circTLCD4-RWDD3}. Scale bars, 100 µm. h–j Representative images (h) and quantification of the Transwell migration (i) and tube formation (j) of circTLCD4-RWDD3^WT and circTLCD4-RWDD3^KO HLECs treated with H1299-EV_Vector or H1299-EV_{circTLCD4-RWDD3}. Scale bars, 100 µm. The statistical difference was assessed with one-way ANOVA followed by Dunnett tests in b–d; and unpaired Student’s t-test in (b, c, f, g, i, and j). Error bars show the SD from three independent experiments. *P < 0.05; **P < 0.01