Fig. 4
From: Antigen-induced chimeric antigen receptor multimerization amplifies on-tumor cytotoxicity
A model for the BCMA–NbT complex. a Superposition of the Nb1 and the Nb2 complexes based on BCMAECD. The secondary interface observed in the Nb1–BCMAECD structure is omitted for clarity. b Nanobody epitopes mapped on the BCMAECD surface. Residues in contact with Nb1 are outlined in blue while those in contact with Nb2 in yellow. c Competition for BCMA binding between Nb1 and Nb2 assayed using SPR. Fc-tagged human BCMAECD was immobilized onto a Protein A sensor chip as the ligand. Nb1 (2000 nM) or Nb2 (100 nM) was loaded with BCMAECD-Fc for 1 min, followed by a mixture of Nb1 (2000 nM) and Nb2 (100 nM). d Small-angle X-ray scattering (SAXS) envelopes and corresponding models of Nb1 (left), Nb2 (middle), and NbT (right) based on the crystal structures of the Nb1–BCMAECD and the Nb2–BCMAECD complexes
