Fig. 2
From: Pyrotinib and chrysin synergistically potentiate autophagy in HER2-positive breast cancer
The role of autophagy in the synergistic effect of chrysin and pyrotinib. a Autophagy flux of SK-BR-3 cells labeled with mRFP-GFP-LC3 in the different treatment groups. b Western blot analysis of the protein expression levels of autophagy markers, LC3-II/LC3-I and P62, in various treatment groups (n = 3). c Fluorescence imaging detection of ER stress in SK-BR-3 cells after receiving various treatments using ER-Tracker staining (n = 3). d, e Western blot (d) and RT-qPCR (e) analysis of expression level of ER stress markers, IRE1 or p-eIF2A, in SK-BR-3 cells after various treatments (n = 3). All bar values are represented as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001
