Fig. 5
From: DNA framework signal amplification platform-based high-throughput systemic immune monitoring
The excellent sensitivity, specificity, and stability of the DSAP. a The comparison between the DSAP and nude HCR probes in detecting membrane protein. CLSM images of HuT-78 cells treated with DSAP and nude HCR probes for 15, 30, and 60 min, respectively. The cell membranes were stained with DiO dye (green channel), and the released fluorescence from DSAP was Cy5. The statistical results of nude HCR probes and DSAP were shown by histogram. b The FCA of HuT-78 cells treated with DSAP and nude HCR probes from 0 to 60 min at 10 min intervals. c The MFI of HuT-78 cells at increasing concentrations after treatment with DSAP and nude HCR probes. The MFI of HuT-78 cells treated with the DSAP and nude HCR probes from 0 to 60 min. d The schematic illustration of signal switching off/on DSAP. The MFI and visualization of CD4+ cells (HuT-78 cells and U937 cells) and CD4− cells (HL-60 cells and A549 cells) of varying concentration after treatment with the DSAP. e The MFI and emission wave curve of the mixture containing HuT-78 cells and HL-60 cells of varying concentration ratios after treatment with DSAP. f CLSM images of HuT-78 cell, CD4+ T lymphocyte, and HL-60 cell, as well as colocalization profiles of Alexa 488 channel and Cy5 channel after treating FAM/Cy5/BHQ-2 labeled DSAP. The CLSM images were overlaid by the Cy5 channel and FAM channel. g The FCA of HuT-78 cell, CD4+ T cell, A549 cell and HL-60 cell treated with FAM/Cy5/BHQ-2 labeled DSAP. The error bars in (a, c–e) are determined by the SD of the MFI from at least three parallel experiments. All the tested samples were technical replicates. The **** represented p < 0.0001, ** represented p < 0.01. Scale bars, 50 μm
