Fig. 6
From: DNA framework signal amplification platform-based high-throughput systemic immune monitoring
The validation of DSAP for diverse immune cells. a The CLSM results of isolated human CD4+ T and CD8+ T lymphocytes treated with CD4 nude HCR probes and DSAP as well as CD8 nude HCR probes and DSAP for 30 and 60 min, respectively. The CD4 DSAP and nude HCR probes were modified with Cy5/BHQ-2, and the CD8 DSAP and nude HCR probes were modified with FAM/BHQ-1. The histogram on the right shows the statistical results from three parallel experiments. b The FCA of whole blood cells treated with CD4 DSAP and CD4 DSAP and CD14. c The CLSM of blood samples treated with both CD4 DSAP (red channel) and CD4 DSAP (green channel). d The MFI of isolated human CD4+ T lymphocytes at increasing concentrations after treatment with the DSAP. e The MFI of isolated human CD8+ T lymphocytes of increasing concentrations after treatment with the CD4 DSAP and CD4 DSAP. The dots in (d) and (e) represent the mean average of MFI from at least three parallel experiments. The gray bands in (d) and (e) represent the 95% confidence interval band. All the tested samples were technical replicates. Scale bars, 50 μm
