Fig. 3

Mice lacking Dok6 in DRG neurons showed peripheral myelin abnormalities. a Schematic representation of the constructs used to create the transgenic mice. b Two kinds of Cre tool mouse information, Sns, and Avil, are specifically expressed in neurons. Sns is mainly expressed in most nociceptors, and Avil has a range of expression and targets all subsets of peripheral sensory neurons. c The ultrastructure of the sciatic nerve observed with TEM in age-matched wild-type control group mice and Avil-Cre; Dok6 ^fl/fl mice at P7. Degenerating myelin (arrowhead) was present in Avil-Cre; Dok6 ^fl/fl mice. Scale bar, 2 μm (panels 3 and 4, Scale bar, 1 μm). d The ultrastructure of the sciatic nerve observed with TEM in control and Avil-Cre; Dok6 ^fl/fl mice at P14. Myelin sheaths with dense degeneration and other abnormal phenotypes were present in Avil-Cre; Dok6 ^fl/fl mice. Scale bar, 2 μm. e The percentage of myelinated axons in the P7 (n = 3 animals per genotype, on average 17 electron micrographs per animal) and P14 (n = 3 animals per genotype, on average 25 electron micrographs per animal) control, Avil-Cre; Dok6 ^fl/fl mice sciatic nerve (n = 3 per genotype). Randomly taken electron micrographs at the same magnification per animal were chosen. f Total axon number in the P7 and P14 control, Avil-Cre; Dok6 ^fl/fl mice sciatic nerve. g Quantitation of sciatic nerve myelin abnormalities in control and Avil-Cre; Dok6 ^fl/fl mice at P7 (n = 3 animals per genotype, on average 17 electron micrographs per animal) and P14 (n = 3 animals per genotype, on average 25 electron micrographs per animal). Randomly taken electron micrographs at the same magnification per animal were chosen. h The ultrastructure of the sciatic nerve observed with TEM in control and Sns-cre; Dok6 ^fl/fl mice at P14. Abnormal myelin sheaths were detected in Sns-cre; Dok6 ^fl/fl mice. Scale bar, 2 μm. Data are presented as the mean ± SD, ***p < 0.001, two-tailed unpaired Student’s t test