Fig. 4
Motor abnormality, nociceptive hypersensitivity, and peripheral myelin abnormalities in Avil-Cre; Dok6fl/fl rather than in Cnp-Cre; Dok6fl/fl mice. a MBP immunostaining (red) in the P7 littermate control, Dok6 mutant, Cnp-cre; Dok6 fl/fl and Avil-Cre; Dok6fl/fl mice sciatic nerve. Scale bar, 10 μm. b The ultrastructure of the sciatic nerve observed with TEM in Control and Cnp-cre; Dok6 fl/fl mice at P14. Scale bar, 10 μm. c–i Motor performance was examined with the grip strength test, beam balance test, coat hanger test, rotarod test, and treadmill test in control, Avil-Cre; Dok6fl/fl and Cnp-Cre; Dok6fl/fl mice at P60. Nociceptive sensitivity was measured by the hot plate test. j, k Conduction velocity recording of CAPs with stimulated right sciatic nerves from Control, Avil-Cre; Dok6fl/fl and Cnp-Cre; Dok6fl/fl mice at P60. Representative traces from measurements per genotype are shown. l, m Nerve conduction velocity and amplitude in control, Avil-Cre; Dok6fl/fl and Cnp-Cre; Dok6fl/fl mice. Data are presented as the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, two-tailed unpaired Student’s t test
