Fig. 5
From: Alpha5 nicotine acetylcholine receptor subunit promotes intrahepatic cholangiocarcinoma metastasis
Acetylcholine/CHRNA5 axis induced axonogenesis through increasing the expression of BDNF in ICC. a, b IHC detection of PGP9.5 in CHRNA5 silencing or overexpressing ICC cells xenografts. c, d Immunofluorescence staining of β3-Tublin-based detection of nerve outgrowth of DRG cocultured with conditioned medium of CHRNA5 silencing or overexpressing ICC cells. e, f qRT-PCR-based detection of BDNF in CHRNA5 silencing or overexpressing ICC cells. g IHC-based detection of PGP9.5, p-CAMKII, β-catenin and Ki67 in YAP/Akt mice treated with ANA-12. h, i qRT-PCR-based detection of BDNF mRNA expression in ICC cells treated with ICG-001. j, k qRT-PCR-based detection of BDNF mRNA expression in CTNNB1 silencing or overexpressing ICC cells. l Multiplex immunofluorescence staining of CK19, PGP9.5, P-CAMK II, P-GSK3β, β-catenin and BDNF. Representative results from at least three experiments are shown. Data are shown as means ± SD. *p < 0.05; **p < 0.005
