Fig. 8
From: Elevated Kallistatin promotes the occurrence and progression of non-alcoholic fatty liver disease
High FFA reverses the downregulation of KAL induced by T3 and Fenofibrate down-regulates KAL in hepatocytes. a Protein levels of KAL in L-02 cells treated with OP (250 μM Oleic acid add 250 μM Palmitic acid) or BSA (Bovine serum albumin) for 24 h. b Protein levels of KAL in L-02 cells treated with T3 (25 nM) for 24 h. c–f Representative immunoblot and quantification of KAL, mRNA levels of KAL, and supernatant KAL levels in L-02 cells treated with T3 (25 nM) with/without OP for 24 h. g–h mRNA levels of KAL, supernatant KAL levels in MIHA cells treated with T3 (25 nM) with/without OP for 24 h. i–k Representative immunoblot of KAL, ATGL, CGI-58 in L-02 cells treated with Metformin (Met), Berberine (BBR), or Fenofibrate (Feno). l Supernatant KAL levels in L-02 cells treated with Feno. m–p Oil red O staining, serum TG level, steatosis score, and the mRNA level of KAL in livers from the rats fed with HFruD for 12 weeks, followed by the treatment of 100 mg/kg Feno and HFruD for 4 weeks. Scale bar: 500 μm. Data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001
