Fig. 3 | Signal Transduction and Targeted Therapy

Fig. 3

From: Mex-3 RNA binding family member A (MEX3A)/circMPP6 complex promotes colorectal cancer progression by inhibiting autophagy

Fig. 3

MEX3A forms a complex with circMPP6 in PBs. a Venn diagram showing the overlap between CRC tissue CircRNA MicroArray, RIP-Seq analysis of Flag-MEX3A, and CLIP-Seq analysis of MOV10 by CircInteractome (https://circinteractome.nia.nih.gov/). b The genomic locus of circMPP6. Left, Sanger sequencing showing the back splicing junction site of circMPP6. Right, PCR products with or without RNase R pre-treatment showing circularization of circMPP6 in DLD-1 and SW480 cells. cDNA, complementary DNA. c RNA fluorescence in situ hybridization (RNA FISH) assay showing the distribution of circMPP6 in DLD-1 and SW480 cells. Nuclei were stained with DAPI (blue). Scale bar, 10 µm. d qRT–PCR analysis of RIP assay verifying the binding of circMPP6 to MEX3A and MOV10 in DLD-1 and SW480 cells. IgG antibody served as the negative control. e Western blotting of circMPP6 Pull-down assay showing the specific association of MEX3A and MOV10 protein with circMPP6 in DLD-1 and SW480 cells. f IF-FISH assays showing that the colocalization of MEX3A/MOV10/circMPP6 was increased after incubation with 20 μg/mL puromycin for 4 h in DLD-1 and SW480 cells. Left, representative images. Nuclei were stained with DAPI (blue). Scale bar, 10 µm. Right, Pearson’s correlation coefficient analysis. Data are represented as mean ± S.D. from three independent experiments (d, f). The P value was determined by a two-tailed unpaired Student’s t test (f)

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