Fig. 2 | Signal Transduction and Targeted Therapy

Fig. 2

From: Thiamine-modified metabolic reprogramming of human pluripotent stem cell-derived cardiomyocyte under space microgravity

Fig. 2

Metabonomic analysis of cell supernatants under space microgravity condition. a The volcano plot showing differential metabolites of cell supernatant under 1 g and μg conditions. b The heatmap plot showing that 3 metabolites were higher in μg cell supernatant, and 10 metabolites were higher in 1 g cell supernatant. c The KEGG analysis of differential metabolites. d Thiamine content in the cell culture supernatant of hPSC-CMs under 1 g and μg conditions. e The relative mRNA expression of SLC19A2 (thiamine transporter) in hPSC-CMs under 1 g and μg conditions. f The protein expression of SLC19A2 in hPSC-CMs under 1 g and μg conditions. g Transcriptomic analysis showing decreased expression profile of genes related to TCA cycles in hPSC-CMs under μg conditions. h The comparison of lactate content in cell culture supernatant of hPSC-CMs under 1 g and μg conditions. Data are presented as mean ± SEM; Student’s t test; *P < 0.05, **P < 0.01

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