Fig. 7 | Signal Transduction and Targeted Therapy

Fig. 7

From: A novel engineered IL-21 receptor arms T-cell receptor-engineered T cells (TCR-T cells) against hepatocellular carcinoma

Fig. 7

Immunological characteristics of IL-21R-TCR-T profiled by scRNA-seq. a Combined UMAP (Uniform Manifold Approximation and Projection) plot of all sequenced cells that passed QC (Quality Control) for subsequent analyses in this study. The color denotes cell types. b Characteristics of the CD8+ T cells clusters as defined by the expression of a panel of functional properties relevant genes. Both color and size indicate the effect value. c Barplot showing the contribution of cell types to each group. d Monocle2 trajectory plot of CD8+ T cells. Cell orders are inferred from the expression of highly variable genes except cell cycle-related genes. Color is coded by CD8+ T cell subpopulations. Insert visualizes the pseudotime defined by Monocle2. e Top, respectively showing memory CD8+ T cells (left) and exhausted T cells (right) in (d). Bottom, cell density of TCR-T and IL-21R-TCR-T along with the pseudotime defined by Monocle 2. f Split violin plot showing the distribution of several immune-related scores of T cells. **P < 0.01, ***P < 0.001, ****P < 0.0001, Mann–Whitney test was used to calculate the significance. The black dots represent the mean score. g Left, Rank for regulons in TCR-T based on regulon specificity score (RSS). The top three regulons are highlighted. Right, same for IL-21R-TCR-T

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