Fig. 7

Restoration of METTL14 function in mice ameliorates liver inflammation, injury, and fibrosis. a Western blot showing METTL14 and GLS2 expression in mouse livers in the WT-NC, KO-NC, and KO-OV groups (n = 4). b, c Relative quantitative analysis of METTL14 (b) and GLS2 (c) expression in mouse livers in the WT-NC, KO-NC, and KO-OV groups. d DCFH-DA was used to display the levels of intracellular ROS in primary cultured hepatocytes isolated from WT-NC, KO-NC, and KO-OV mouse livers (n = 3, scar bar = 50 μm). e Relative quantitative analysis of ROS levels in primary cultured hepatocytes isolated from WT-NC, KO-NC, and KO-OV mouse livers. f TAC levels showing the total antioxidant capacity of mouse livers using ABTS methods in the WT-NC (n = 4), KO-NC (n = 5), and KO-OV (n = 5) groups. g Representative immunofluorescence images displaying the distributions of S100A4-positive, F4/80-positive, and S100A4 F4/80 double-positive macrophages among liver sections in the WT-NC, KO-NC, and KO-OV groups (scar bar = 50 μm or 5 μm). h Relative quantitative analysis of S100A4 F4/80 double-positive macrophages in the WT-NC (n = 4), KO-NC (n = 5), and KO-OV (n = 5) groups. FOV, field of view. i–l ELISA array detecting the levels of 8-OHdG (i), FGF2 (j), MMP2 (k), and S100A4 (l) in mouse liver tissues from the WT-NC (n = 4), KO-NC (n = 5), and KO-OV (n = 5) groups. m Immunofluorescence staining detecting α-SMA expression (left), Sirius Red staining (middle) and Masson staining (right) displaying the activation of HSCs and the severity of fibrosis of liver sections in the WT-NC (n = 4), KO-NC (n = 5), and KO-OV (n = 5) groups (scar bar = 20 μm or 25 μm). Data are represented as mean ± SEM. NS not significant, *p < 0.05, **p < 0.01, *** p < 0.001