Fig. 8
Restoration of GLS2 ameliorates liver inflammation, injury, and fibrosis in Western diet/CCl4-treated mice. a Schematic diagram of the dietary feeding scheme. Six-week-old C57/BL6 wild-type mice were fed a Western diet (WD) for 16 weeks and also treated with CCl4 intraperitoneal injection. Mice fed a WD for 4 weeks were randomly injected with AAV-8 overexpressing Gls2 (OE-AAV) or control vector (CTL-AAV) through the caudal vein (n = 15). b, c Western blot (b) and quantitative analysis (c) showing the expression of GLS2 in the OE-AAV and CTL-AAV mice (n = 12). d Body weight changes of OE-AAV and CTL-AAV mice (n = 15). e Comparison of the liver-to-body weight ratio of OE-AAV and CTL-AAV mice (n = 12). f Representative image of resected livers from OE-AAV and CTL-AAV mice (n = 12). g Serum ALT, AST, TBIL, TG and CHO in OE-AAV and CTL-AAV mice (n = 12). h Tissue ROS analysis of liver sections from OE-AAV and CTL-AAV mice (n = 4, scar bar = 50 μm). i Total antioxidant capacity analysis of OE-AAV and CTL-AAV mice (n = 12). j ELISA analysis showing serum S100A4 levels of OE-AAV and CTL-AAV mice (n = 12). k HE, Oil Red O, Sirius Red, Masson staining and α-SMA immunofluorescence analysis of OE-AAV and CTL-AAV mice (n = 12, scar bar = 20 μm). l QRT-PCR analysis showing the expression of fibrosis markers such as Acta2, Col1a1 and Mmp2 of OE-AAV and CTL-AAV mice (n = 8). Data are represented as mean ± SEM. NS not significant, *p < 0.05, **p < 0.01, ***p < 0.001. The mouse image was created with BioRender.com
