Fig. 2 | Signal Transduction and Targeted Therapy

Fig. 2

From: Cholangiocarcinoma combined with biliary obstruction: an exosomal circRNA signature for diagnosis and early recurrence monitoring

Fig. 2

The discovery of a triple-circRNA panel in both bile and serum exosomes. The isolated exosomes were confirmed by a TEM, b NTA, and c immunoblotting. The main band of TSG101 was indicated with an arrowhead. d Volcano plot of microarray analysis revealed the pattern of differentially enriched exosomal circRNAs between CCA-BO and BBO patients. e Heatmap of six candidate circRNAs detected by microarray analysis. f Quantification of six candidate circRNAs with divergent primers in the discovery cohort. Three of them (hsa-circ-0000367, hsa-circ-0021647, and hsa-circ-0000288) were found to be upregulated in both bile and serum exosomes. g RNase R digestion, and h Sanger sequencing verified the qPCR products. *P < 0.05; **P < 0.01; ***P < 0.001; \({{\rm{n}}.{\rm{s}}.\atop }{\rm{not}}\;{\rm{significant}}\); (Mann–Whitney U test for continuous variables, presented as median with range and quartile)

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