Fig. 1
From: PARP1-DNA co-condensation: the driver of broken DNA repair
The mechanistic mode of PARP1-DNA co-condensation as the driver of broken DNA repair. The mechanistic model for the hierarchical assembly of DNA double-strand break (DSB) condensates to illustrating DNA end synapsis and the recruitment of effector proteins for DNA damage repair. Firstly, poly (ADP-ribose) (PAR) polymerase 1 (PARP1) dimerization and multimerization along DNA mediates co-condensate assembly. Then, rigid PARP1-DNA co-condensates generate forces and mediate synapsis of broken DNA ends. The condensates become enzymatically active and PARP1 PARylation destabilizes condensates. Finally, FET proteins stabilize condensates against dissolution, allowing for the entry of repair factors. (Fig. 1 is adapted from graphic abstract1)
