Fig. 1
Characterization of cross-reactive nanobody N235 to multiple variants. a The binding kinetics of N235 to the NTDs from multiple variants were obtained using a Biacore 8 K system in single-cycle mode. Omicron sub-variants with identical NTD amino acid sequences are represented by the same binding curves. b Kinetic and affinity values (ka, kd, and KD) are the mean ± standard deviation (s.d.) of three independent results. The absence of annotated kd and KD represents no detectable dissociation from the NTDs. The dashed line underneath represents the raw data, and the kinetic fit is shown as a solid line. c Neutralization of pseudotyped variants by N235 in vero cells in vitro; n = 3 per dilution. Experiments were repeated independently three times with similar results and one representative curve is displayed. The IC50 values (μg/mL) are presented as the mean ± s.d. of three independent results
