Fig. 5

The effect of N235 on disrupting S trimer assembly. a Gel filtration profile of BA.2S proteins with and without N235. The BA.2S and N235 proteins elute as single monomer peaks in the gel filtration curves. The BA.2S/N235 complex displays a shifted complex peak. All the samples were assessed by SDS–PAGE. b 2D classifications of free BA.2S proteins, with the sample derived from the peak 4 as depicted in (a). c 2D classifications of complex protein of BA.2 S and N235, with the sample derived from the peak 1 as depicted in (a)