Fig. 2
PIK3CA-related capillary venous malformations in PIK3CATie2-CreER mice only partially signal through AKT proteins. a Kaplan–Meier survival curves of PIK3CAWT, PIK3CATie2-CreER, PIK3CAAKT1KO, PIK3CAAKT2KO and PIK3CAAKT1AKT2-KO mice (n = 20 per group). b Coronal whole-body T2 weighted magnetic resonance images (MRI) of PIK3CAWT, PIK3CATie2-CreER, PIK3CAAKT1KO, PIK3CAAKT2KO and PIK3CAAKT1AKT2-KO mice 6 weeks after Cre recombination. Volumetric quantification of the vascular malformations (n = 4–6 mice per group). c Representative hematoxylin and eosin (H&E) staining of the skin of PIK3CAWT, PIK3CATie2-CreER, PIK3CAAKT1KO, PIK3CAAKT2KO and PIK3CAAKT1AKT2-KO mice. Scale bar: 10 μm. d Representative P-AKTSer473 in the skin of PIK3CAWT, PIK3CATie2-CreER, PIK3CAAKT1KO, PIK3CAAKT2KO and PIK3CAAKT1AKT2-KO mice. Scale bar: 10μm.*: Vascular malformation. e Western blot and quantification of P-AKTSer473 and P-S6RP in the skin of PIK3CAWT, PIK3CATie2-CreER, PIK3CAAKT1KO, PIK3CAAKT2KO and PIK3CAAKT1AKT2-KO mice (n = 4–5 mice per group). f Representative immunofluorescence staining of KI67 in the skin of PIK3CAWT, PIK3CATie2-CreER, PIK3CAAKT1KO, PIK3CAAKT2KO and PIK3CAAKT1AKT2-KO mice. Scale bar: 10 μm. g Flow cytometry experiments showing the percentage of GFP + CD31+ cells isolated from the skin of the different mouse models expressing KI67 (n = 3–4 mice per group). (n = 3–7 mice per group). h Western blot and quantification of AKT1 and AKT2 in vessels of PIK3CAWT, PIK3CATie2-CreER and PIK3CAAKT1AKT2-KO mice (n = 3–4 mice per group)
