Fig. 7
Inhibition of HSP90 attenuates the virulence of SARS-CoV-2 in the infection mouse model. a C57BL/6-hACE2 mice were intraperitoneally injected with 0, 5, or 25 mg/kg 17-AAG for 8 consecutive days. On the second day of drug treatment, the mice were nasally infected with SARS-CoV-2. b Daily recordings of mouse body weights commenced at the time of infection. c Lung tissues were collected seven days post-infection, lysed, and subjected to viral titer analysis using FFA. d Total RNA was extracted from mouse lung tissues, and relative levels of SARS-CoV-2 S and N genes were detected. e The levels of SARS-CoV-2 N protein in the lungs of infected mice treated with 17-AAG or DMSO were assessed by Immunohistochemistry with the anti-SARS-CoV-2 N antibody. f SARS-CoV-2 N proteins and ORF9b proteins in mouse lung tissues were detected by Western blot. g H&E staining of mouse lung tissues was performed to visualize the extent of inflammatory infiltration. h Relative RNA abundance of indicated cytokines or inflammatory factors in mouse lung tissues was measured by qRT-PCR. Quantification was shown as mean ± s.d. n = 4 independent experiments. Student’s t-test (unpaired, two-tailed) was used to compare two independent groups, and a two-way ANOVA test was performed for comparisons of multiple groups. *P < 0.05; **P < 0.01; ***P < 0.001
