Fig. 5 | Signal Transduction and Targeted Therapy

Fig. 5

From: Synergistic induction of mitotic pyroptosis and tumor remission by inhibiting proteasome and WEE family kinases

Fig. 5

BTZ and PD0166285 shows synergistic effect in killing cancer cells but not immortalized cells. a–g Various cancer cell lines were sensitive to BP-Combo treatment. h–l Immortalized cell lines and normal cells were resistant to BP-Combo treatment. Cancer cell lines from hepatoma (SNU449, Huh1, HepG2, Hepa1-6), cervical cancer (HeLa) and osteosarcoma (U2OS), transformed human bronchial epithelial cell line (HBERST), immortalized cell lines (HBE, 293T, L02, LX2) and normal cell (SF) were treated for 48 h with a combination of BTZ and PD0166285 at the indicated concentration. Cell survival was measured by Alamar Blue assay. Cooperativity screens (upper panels) and Loewe plots (down panels) for the synergistic effect of BTZ and PD0166285 are shown based on at least three independent experiments. In upper panels, color bars indicate the percentage of surviving cells in BP-Combo-treated group, which was normalized to untreated group. In down panels, color bars indicate synergy score in the Lowe plots; a score greater than 0 indicates synergism, and less than 0 indicates antagonism

Back to article page