Fig. 2
From: In vivo evaluation of guide-free Cas9-induced safety risks in a pig model
Transcriptome sequencing and whole-genome sequencing of PBMCs from DIC pigs after two weeks of Dox feeding. a Schematic diagram showing Cas9 expression induced by Dox followed by transcriptome or genome sequencing in DIC pigs. b Principal component analysis of RNA-Seq samples from the PBMCs of the DIC and WT pigs after administration of Dox for 0 (D0) and 14 days (D14). c The MA plot shows the up- and downregulated differentially expressed genes in the PBMCs of the DIC pigs after administration of Dox for 14 days. Red points represent the upregulated differentially expressed genes (log2FoldChange > 1 and p-adjusted-value < 0.05), blue points represent the downregulated differentially expressed genes (log2FoldChange < 1 and p-adjusted-value < 0.05), and black points indicate genes with no significant difference in expression. d Hierarchical clustering heatmap (left) and results of KEGG pathway enrichment analysis (right) of the differentially expressed genes from the PBMCs of the DIC and WT pigs after administration of Dox for 0 and 14 days. e Heatmap showing the relative expression of genes related to genomic damages, apoptosis, cell senescence and the P53 signaling pathway. The Z score was calculated to indicate relative expression between samples. f The bar plot shows the number of indels and SNVs detected in the PBMCs of DIC and WT pigs after administration of Dox for 14 days, with background mutations excluded. Data are presented as mean ± SEMs. g The Circos plot shows the whole genome distributions of the detected mutations in the PBMCs of DIC and WT pigs
