Fig. 1 | Signal Transduction and Targeted Therapy

Fig. 1

From: Palmitoylation regulates myelination by modulating the ZDHHC3-Cadm4 axis in the central nervous system

Fig. 1

Cadm4 is specifically palmitoylated at cysteine-347. a Cadm4 expressed in N2a cells or from mouse brain was analyzed for protein palmitoylation by Acyl-RAC assay, and quantified. HA+, with hydroxylamine, HA−, without hydroxylamine. b, c N2a cells expressing Cadm4-Flag was incubated with 2-BP for various dose and time, and were evaluated for the level of palm-Cadm4, and quantified (n = 3 biological replicates, one-way ANOVA; *p ≤ 0.05, **p ≤ 0.01, ****p ≤ 0.0001, n.s. for not significant). d Protein sequences of Cadm4 from various species were aligned for analyzing cysteine conservation. e Cadm4 or its mutants (C347A and C339A/C347A) were expressed in N2a cells and subjected for Acyl-RAC assay, and quantified (two-tailed t-test, n = 3, ****p ≤ 0.0001). Cadm4 and its mutants (C347A and C339A/C347A) expressed in N2a cells (f) or endogenously expressed Cadm4 (g) were processed with mPEG-labeling assay. The mPEG labeling causes the band shift, red arrows pointed. Data are represented as mean ± SEM

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