Fig. 2

Palmitoylation regulates Cadm4 for dynamic PM localization. a N2a cells expressing either Cadm4 or Cadm4-C347A were fixed for immunofluorescence analysis, Na/K-ATPase is a marker for PM. b Intensity of the red and green fluorescence was profiled along the slash. c N2a cells expressing Cadm4 or Cadm4-C347A were treated with DMSO or 2-BP for preparing plasma membrane fractions, which were evaluated by WB and quantified (one-way ANOVA followed by Bonferroni post hoc test, n = 3, **p = 0.009; ***p = 0.001). d N2a cells expressing Cadm4 or Cadm4-C347A were treated with DMSO or Dynasore for preparing plasma membrane fractions, which were evaluated by WB and quantified (two-tailed t-test, n = 3, **p ≤ 0.01). Live-cell imaging of N2a cells expressing Cadm4-GFP/Cadm4-C3471-GFP with RFP-Rab5 (e), for colocalization analysis (f). Data are represented as mean ± SEM