Fig. 3

Internalized depalmitoylated Cadm4 is targeted for protein degradation. a Cadm4 or Cadm4-C347A was expressed in N2a cells, treated with DMSO or 2-BP (50 µM) and fixed for immunofluorescence analysis, Lamp1 is a marker for lysosome. b The colocalization of Cadm4/Lamp1 was quantified (one-way ANOVA followed by Bonferroni post hoc test, n = 5, ***p < 0.001). N2a cells expressing Cadm4 or Cadm4-C347A were incubated with CHX (cycloheximide, 100 µM) for various length of time, lysates of which were subjected for WB analysis (c) and the levels of Cadm4 were quantified (d). **p = 0.007, paired t-test, n = 3. N2a cells expressing Cadm4 treated with DMSO or 2-BP (50 µM) were incubated with CHX for different periods, analyzed by WB (e) and the levels of Cadm4 were quantified (f). **p = 0.018, paired t-test, n = 3. N2a cells expressing Cadm4 or Cadm4-C347A were incubated with or without CHX, CHQ (Chloroquine, 50 µM), 3-MA (Methyladenine, 3 mM) or Mg132 (25 µM), and subjected for WB analysis (g), and quantified (h). Data are represented as mean ± SEM