Fig. 2 | Signal Transduction and Targeted Therapy

Fig. 2

From: Corynebacterium parakroppenstedtii secretes a novel glycolipid to promote the development of granulomatous lobular mastitis

Fig. 2

C. parakroppenstedtii causes GLM in rats. a Images of C. parakroppenstedtii. Left: typical colonies of C. parakroppenstedtii in BHIY with Tween 80. Middle: microscopic view of C. parakroppenstedtii. Right: C. parakroppenstedtii observed by scanning electron microscopy. b Genome circle map of C. parakroppenstedtii P1. From outside to inside, coding genes (positive-sense strand), coding genes (negative-sense strand), tRNA (orange) and rRNA (purple), gene islands (green), GC ratio, GC skew, and sequencing depth. c C. parakroppenstedtii P1 causes changes in cytokine levels in MCF-10A cells. Three biological replicates were set up for each group. Data are presented as the mean values ± standard deviation (SD), and Kruskal–Wallis tests were performed to calculate statistical significance. d Verification of the occurrence of GLM after C. parakroppenstedtii P1 infection. The experiment consisted of a control (phosphate-buffered saline [PBS]) group (12 rats) and a C. parakroppenstedtii P1 group (12 rats). After breast fat pad injection, six rats in each group were sacrificed on day 3 and day 10. Pathologic results are typical representative images selected from the supplementary fig. 2. Scale bar represents 100 μm. e The serum cytokine levels in the rats were determined using a Luminex assay. Data were compared between the rats with obvious inflammation (day 3: n = 4; day 10: n = 5) and those in the control group (n = 6). Data are presented as the mean values ± standard error of the mean (SEM), and Kruskal–Wallis tests were performed to calculate statistical significance. f The levels of cytokines in the breast tissue of rats were determined by immunofluorescence staining. Data were compared between the rats with obvious inflammation (day 3: n = 4; day 10: n = 5) and those in the control group (n = 6). Data are presented as the mean values ± SEM, and Kruskal–Wallis tests were performed to calculate statistical significance. g Verification of the occurrence of GLM after infection with different C. parakroppenstedtii strains or the blank control (PBS). There were five rats in each group. The results were observed after sacrifice on day 10 after breast fat pad injection. h Antimicrobial susceptibility testing of C. parakroppenstedtii with different antibiotics performed via disk diffusion (Becton Dickinson Deutschland, Heidelberg, Germany) as suggested for Corynebacterium by the European Committee on Antimicrobial Susceptibility Testing (http://www.eucast.org)

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