Fig. 4

Statistical differences in immune cells among three groups. a Statistical graph of immune cells in liver cancer cells. b Left: Statistics of fibroblasts; Right: fibrotic barrier formed between tumor tissue and necrotic tissue, with the image mass cytometry (IMC) image on the top showing Pan-cytokeratin (cyan), Collagen I (blue), CD31 (red), scale bar, 100 μm; and the immunohistochemistry image of Pan-cytokeratin at the bottom, scale bar, 300 μm. c Statistics of endothelial cells on the left, with the representative IMC image of endothelial cells on the right, scale bars, 80 μm. d Statistical graph of various immune cell clusters. e Left: Statistics of CD4 + T cells with B cells in TLS; Right: Representative IMC image of TLS, with CD20 + B cells (cyan), CD4 (blue), CD3 (red), CD4 + T cells represented in pink after overlap of CD4 and CD3, Pan-cytokeratin (green), CD31 (yellow), TLS outlined by white dashed lines. Scale bars, 100 μm. f Statistical graph of neutrophils