Fig. 4 | Signal Transduction and Targeted Therapy

Fig. 4

From: Human adipose and umbilical cord mesenchymal stem cell-derived extracellular vesicles mitigate photoaging via TIMP1/Notch1

Fig. 4

AMSC-EV and HUMSC-EV mitigated photoaging in constructed full-thickness skin model. a Schematic representation of establishment and treatment of T-Skin model (Created with BioRender.com). b Representative images of histological analysis and biomarker staining of the T-Skin model (scale bar, 15 μm). c Representative images of SA-β-gal staining (scale bar, 20 μm). d Quantitation of SA-β-gal positive cells. n = 3, **P < 0.01, ***P < 0.001, ****P < 0.0001. e Representative images of ROS staining (scale bar, 20 μm). f Quantitation of ROS-positive cells. n = 3, ****P < 0.0001. g Representative images of γ-H2Ax staining (scale bar, 20 μm). h Quantitation of γ-H2Ax positive cells. n = 3, **P < 0.01, ****P < 0.0001. i Representative images of MSC-EV uptake by T-Skin model after 48 and 72 h (left, scale bar, 150 μm; right, scale bar, 25 μm). j Quantitation of IL-1β, IL-6, and TNF-α released by T-Skin model was detected through ELISA. n = 3, ****P < 0.0001

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