Fig. 7

MSC-EV rescues HDFs and HaCaTs photoaging by upregulating TIMP1. a Venn diagram of the protein contents of AMSC-EV and HUMSC-EV. b Heatmap of the protein contents of AMSC-EV and HUMSC-EV. c Heatmap of extracellular matrix organization. d Western blot analysis showing COL1, COL3, COL6, and TIMP1 expression in AMSC-EV and HUMSC-EV. e Quantitation of TIMP1 in AMSC-EV and HUMSC-EV was detected by Western blot. n = 3. f Representative immunofluorescence staining images of positive cells of ROS (green) and DAPI (scale bar, 35 μm). g Quantitation of ROS-positive cells. n = 3, ****P < 0.0001. h Representative immunofluorescence staining images of positive cells of γ-H2Ax (red) and DAPI (scale bar, 20 μm). i Quantitation of a mean number of γ-H2Ax foci/cell. n = 3, ***P < 0.001, ****P < 0.0001. j Representative images of SA-β-gal staining in HDFs and HaCaTs (scale bar, 100 μm). k Quantitation of SA-β-gal positive cells in HDFs and HaCaTs. n = 3, ***P < 0.001, ****P < 0.0001. l Representative images of transwell assays of HaCaTs (scale bar, 100 μm). m epresentative images of migration assay of HaCaTs (scale bar, 100 μm). n Western blot analysis showing the change of COL3, TIMP1, MMP1 and MMP9 in HDFs