Fig. 6

Spatial cooperation of combination therapy eradicates advanced-stage melanoma. a Therapeutic schedule of advanced-stage melanoma treatment (n = 7–8 biologically independent animals). b Tumor growth curves and statistical analysis of tumor volume for different treatment groups. c Body weight change curves post-treatment. d Representative images of tumor-bearing mice after different treatments. e Photographs and tumor weight analysis of the harvested tumors from different treatment groups, scale bar = 1 cm. A dotted circle indicates a single mouse in which the tumor was eradicated. f Representative H&E images of tumor sections of different treatment groups, scale bar = 1 mm. g Representative fluorescence intratumor staining of Ki-67, scale bar = 1 mm. h Representative immunofluorescent intratumor staining of TUNEL, scale bar = 1 mm. i Representative intratumor staining of CD3 and E. coli, scale bar = 1 mm. Data are shown as mean ± s.d. P values were determined by one-way ANOVA with a Tukey post hoc test