Fig. 5

Transcriptomic and multiplexed immunofluorescence analyses on samples from long benefit versus early progression groups. a Volcano plots showing the expression profiles of cytokines. b Protein interaction mapping of differentially expressed cytokine. c GSEA enrichment analysis with Hallmarker gene sets. d Proportion comparison of 22 immune cells infiltration abundance between long benefit and early progression groups. e Heatmap of 22 immune cells in long benefit and early progression groups. f Comparisons of 22 immune cells infiltration abundance between long benefit and early progression groups. g Multi-color immunofluorescence staining of CD66b⁺, CD8⁺, PD-1⁺, PD-L1⁺, CXCR1⁺ and PanCK⁺ cells between long benefit and early progression groups. h The comparisons of CD66b, CD8, PD-1, PD-L1, CXCR1 and PanCK expression between long benefit and early progression groups. i The umap plot of neutrophil between long benefit and early progression groups. j The proportion of each subgroup between long benefit and early progression groups. ns, P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001