Fig. 3

Patients’ responses by single-cell analysis of matched peripheral blood samples from patients collected at 1 day before and 7 days after UCB infusion. a Uniform manifold approximation and projection (UMAP) plot depicting the 23 identified cell types. Each dot represents an individual cell. Cell populations are coded by different colors. CD4T CD4⁺ T cells, CD8T CD8⁺ T cells, NKT nature killer/T cells, NK natural killer cells, Mo monocyte cells, DC dendritic cells, HSPC hematopoietic stem and progenitor cells. b Stacked bar blot showing the proportions of the 23 identified cell types across each sample. The colors indicate different cell populations as same as panel a. P represents patients, b represents before UCB infusion and a represents after UCB infusion. c Box plot illustrating the dynamic changes in cell fractions of cytotoxic T cells (CD8T_01_GZMK, CD8T_06_STMN1), NK cells (NK_02_XCL1) and monocytes (Mo_01_CD14). The colors represent different patients and time points. * denotes statistical significance (P < 0.05) performed by using the Wilcoxon rank sum test. d Dot plot visualizing the enriched GO terms of up-regulated DEGs in different cell populations post-UCB compared to pre-UCB infusion. The size of nodes represents the ratio of DEGs that are annotated in each term to all genes in that term. Colors represent the adjusted one-sided Fisher’s exact test P values by Benjamini-Hochberg correction