Fig. 1

The importance of the unstructured linker of lipoprotein for efficient lipoprotein extraction by LolCDE. a A Schematic diagram of lipoprotein transportation by LolCDE from IM to OM. b Density map (left) and cartoon representation (right) of the lipoprotein-bound LolCDE structure in nanodiscs. LolC and LolE are shown in purple and green, LolD dimer is shown in pink and yellow, and lipoprotein is shown in red, respectively. c Cartoon representation of BamE structure (PDB code: 2KM7), indicating the truncation positions of the unstructured linker. d Substrate binding analysis of the truncated BamE by LolCDE. BamE was truncated from the N-terminus (left) or C-terminus (right) of the unstructured linker. e Residues of LolE surrounding the unstructured linker of lipoprotein and corresponding residues in LolC. f, g In vitro photo-crosslinking of lipoprotein with LolE (f) and LolC (g). Data in d, f, and g are representative of n = 3 independent experiments