Fig. 3
From: Deciphering the molecular basis of lipoprotein recognition and transport by LolCDE
Conformational change of the TM2 periplasmic neck of LolC and LolE in lipoprotein- and ATP-bound structures. a Structural superimposition of the lipoprotein-bound and ATP-bound LolCDE structures reveals conformational changes between TM helices of LolC and LolE. b Residues of the TM2 periplasmic necks of LolC and LolE are indicated. c A pair of tryptophan at the bend of the TM2 periplasmic neck of LolC and LolE, showing multiple contacts with surrounding residues in LolC (left) and LolE (right). d Cell viability of the mutants shown in (b). e Detections of the lipoprotein BamE binding to the mutant complexes shown in (d) by western blotting. Data in d, e are representative of n = 3 independent experiments
