Fig. 4
From: Deciphering the molecular basis of lipoprotein recognition and transport by LolCDE
Analysis of lipoprotein export path between the periplasmic domains of LolCDE. a The hydrophobic cavity formed by the periplasmic cores of LolC and LolE in the ATP-bound structure of LolCDE. The hairpin loop of LolCE and the hook of LolE are highlighted with the indication of the residues mutated for the disulfide-bonded crosslink. b Cell viability of the mutants shown in (a). c In vitro lipoprotein transport assay of the disulfide-bonded mutants LolCV111CDEA112C and LolCA106CDEH177C to LolA. Data in b, c are representative of n = 3 independent experiments. d Conformational changes of the hooks of LolC and LolE in the lipoprotein-bound and ATP-bound structures
